Fig. S10.

Analysis of static lengths in dimeric VWF constructs. Characteristic lengths were determined from height profiles of individual dimers, obtained by tracing each dimer in AFM images individually along its contour, following local maxima in height. Arrows indicate the position of the CK domains, which were identified with the last C-terminal local maximum in height. The beginning of the higher N-terminal domains was defined as the position where the first large increase in height N-terminal of the CK domains reached its half-maximum value. (Scale bar, 30 $\text{nm}$; range of color scale, 2.4 $\text{nm}$.) (A) Analysis of wild-type dimers. (B) Analysis of dimers lacking the D4 domain.