Fig. S11.

Distributions of the normalized stem length of dimers lacking the D4 domain (A) and wild-type (WT) dimers adsorbed from EDTA buffer (B). Both distributions show a single peak decaying from zero stem length. To estimate the interaction free energy $\mathrm{\Delta }G$ between opposite C domains, a simple model for C-terminal stem formation was fitted to both distributions. The fit yielded an interaction free energy $\mathrm{\Delta }G=0.49$ $k_{\text{B}}T$ for delD4 dimers and $\mathrm{\Delta }G=0.56$ $k_{\text{B}}T$ for WT dimers adsorbed from EDTA buffer (see SI Results).