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. 2016 Jan 14;113(5):1273–1278. doi: 10.1073/pnas.1500992113

Fig. S1.

Fig. S1.

UPAT (NR_002773) is required for the survival of colon cancer cells. (A) qRT-PCR analysis of the expression of NR_015379 and UPAT (NR_002773) in CCSC#P and CCSC#11 cells. Results are expressed as the mean ± SEM (n = 3). *P < 0.05. (B) CCSC#P and HCT116 cells were transfected with siRNAs targeting the indicated genes and subjected to qRT-PCR analysis. Results are expressed as the mean ± SEM (n = 3). (C) Viability of CCSC#P and HCT116 cells transfected with siRNAs targeting NR_015379 and UPAT (NR_002773) was assessed by Cell Titer-Glo assays. Results are expressed as the mean ± SEM (n = 3). (D–F) UPAT is homologous to AOC2 and AOC3 but not to AOC1. (D and E) Alignment between UPAT and AOC2 (D) or AOC3 (E). (F) Phylogenetic trees of the AOC family of genes and UPAT. (G) Expression levels of UPAT in colon cancer and normal cell lines. UPAT expression was quantitated as the percentage relative to β-actin mRNA. (H) UPAT is localized in the nucleus. HCT116 cells were subjected to subcellular fractionation, and the amounts of UPAT in each fraction were evaluated by qRT-PCR. α-tubulin and GAPDH mRNA was used as a marker specific for the cytoplasm. Lamin A/C and U1 small nuclear RNA was used as a nuclear marker. Results are expressed as the mean ± SEM (n = 3).