Fig. 5.

LRP1 regulates NFκB activation. (A) BMDMs from C57BL/6J mice were treated with 150 nM RAP for the indicated times. (B) BMDMs were treated with vehicle (C), LPS (0.1 µg/mL), LF (150 nM), or RAP (150 nM) for 30 min. (C) The same incubations were conducted for 8 h. Immunoblot analysis was performed to detect phospho-IκB and total IκB. (D and E) BMDMs were pretreated with JSH-23 (10 μM), LY294002 (20 μM), or vehicle for 16 h and then with LF, RAP, or vehicle (C) for 8 h. mRNA levels were determined for TNFα and CCL3 (mean ± SEM; n = 6; **P < 0.01, ***P < 0.001; one-way ANOVA with Tukey’s post hoc test). (F) BMDMs were treated with El-tPA (12 nM) or α₂M* (10 nM) together with LPS (0.1 µg/mL) or with LPS or vehicle (C) alone for 1 h. Immunoblot analysis was performed. (G) The same experiment was performed for 8 h.