Fig. S4.

Asr1 and Ubp3 control expression of a euchromatic gene also regulated by Sir2. Total RNA was extracted from yeast cells of the indicated genotype (WT, BY4741; ∆asr1, YTM5; asr1^RINGm, YTM27; ∆ubp3, ∆Ubp3; ∆asr1∆ubp3, YTM6; asr1^RINGm∆ubp3, YTM28; ∆sir2; ∆Sir2), and RT–QPCR was used to measure transcript levels from the indicated loci. ACT1 was used as control locus. The expression level of each gene in WT (WT) congenic cells was set to one. Error bars represent SEM (n = 7). See Table S2 for strain details.