Skip to main content
. 2016 Jan 19;113(5):E538–E547. doi: 10.1073/pnas.1520045113

Fig. S3.

Fig. S3.

The deacetylase-dead SIRT6 mutant (SIRT6H133Y) cannot repress PKM2 functions. (A) HepG2 SIRT6kd (HepG2 SIRT6kd) cells were subjected to glucose starvation for 36 h and were infected with adenovirus expressing SIRT6 (Ad-SIRT6) or adenovirus expressing the deacetylase-dead SIRT6 mutant (Ad-SIRT6H133Y) during the last 24 h of the 36-h period, as indicated. The cells then were harvested, and Western blotting was performed for the indicated proteins. (B) HepG2 SIRT6kd (HepG2 SIRT6kd) cells were subjected to glucose starvation for 36 h and were infected with Ad-SIRT6 or Ad-SIRT6H133Y during the last 24 h of the 36-h period, as indicated. The cells then were harvested, and Western blotting was performed for the indicated proteins. (C) HepG2 SIRT6kd (HepG2 SIRT6kd) cells were subjected to glucose starvation for 36 h and were infected with Ad-SIRT6 or Ad-SIRT6H133Y during the last 24 h of the 36-h period as indicated. The cells then were harvested, and nuclear extracts were subjected to immunoprecipitation using anti-PKM2 antibody. Western blotting was performed for the indicated proteins.