Fig. S1.

Partial sequence alignment of the microchimeric GluClR subunits used in this study. An asterisk indicates identical amino acids. A colon indicates groups of strongly similar properties. A period indicates groups of weakly similar properties. Colors match the colors used in Fig. 1. GluClα_cryst is a truncated α subunit used by Hibbs and Gouaux (23) for structure determination by X-ray crystallography (PDB ID code 3RIF). GluClαWT is the WT α subunit of a GluClR from C. elegans (entry code G5EBR3 in the UniProtKB database). GluClβWT is the WT type β subunit of a GluClR from C. elegans (entry code Q17328 in the UniProtKB database). GluClβ_α-CysL, GluClβ_α-β8β9L, and GluClβ_α-Loops are three microchimeric β subunits where the Cys loop, β8β9 loop, or both loops were replaced by the homologous loops of the GluClα subunit, respectively.