Fig. S4.

Pairwise sequence alignment used for 3D homology modeling of the GluClβ subunit. Sequence alignment between a truncated GluClβ subunit lacking the M3–M4 connecting segment (GluCl-beta-tr.) and the truncated GluClα subunit used for expression, crystallization, and X-ray crystallography of the homomeric GluClα_crystR (PDB ID code 3RIF) (23). The α-helices and β-strands of the GluClβ subunit 3D homology model are indicated with the gray letters “h” and “s,” respectively. An asterisk indicates identical amino acids. A colon indicates groups of strongly similar properties. A period indicates groups of weakly similar properties.