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. Author manuscript; available in PMC: 2016 Nov 15.
Published in final edited form as: Bioorg Med Chem. 2015 Nov 15;23(22):7226–7233. doi: 10.1016/j.bmc.2015.10.019

Fig. 2.

Fig. 2

1-Benzyl-2-methyl-3-indolylmethylene thiobarbiturate compounds induce apoptosis in mutant NPM1 expressing leukemia cells: A. OCI-AML3 cells were treated with the indicated concentrations of 1-benzyl-2-methyl-3-indolylmethylene thiobarbiturate compounds (7i, 7j, 7k, and 7l) and YTR-107 (1) for 48 hr. B. OCI-AML3 cells were treated with the indicated concentrations of compound 7k for 48 hr. C. OCI-AML3 cells were treated with the indicated concentrations of compound 7l for 48 hr. After the indicated incubation time period, cells were stained with annexin V and TO-PRO 3 and the percentages of apoptotic cells were measured by flow cytometry. Columns represent the mean of 3 independent experiments; bars represent the SEM.