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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Immunol Cell Biol. 2015 Aug 18;94(2):213–219. doi: 10.1038/icb.2015.72

Figure 2. Galectin-8 binds α2,3-sialylated ligands on CD4+ T cells and promotes Ag-specific Treg cell differentiation.

Figure 2

(A) Splenic CD4+ T cells were stimulated with plate-bound anti-CD3 and anti-CD28 mAbs under Treg polarizing conditions for 4 d in the presence of 1.5 μM Gal-8. TDG and α2,3-sialyllactose were used as galectin inhibitors. (B) CD4+ T cells were isolated from OT-II mice and cultured under Treg polarizing conditions with irradiated congenic splenocytes in the presence of OVA323–339 and Gal-8, as indicated. Relative represents percentage of marker positive cells in gated population as compared to control, and % of max represents the total number of cells at a given fluorescence intensity compared to the maximum number of cells at any intensity for a given sample. Error bars represent mean ± SEM of three independent experiments. P values were determined by ANOVA with Tukey’s multiple comparison test. *, p < 0.05;**, p < 0.01. Significance in panel A is compared to Treg cell polarization in the presence of Gal-8.