Table 1.
Antibodies used for immunohistochemical studies performed in the present study.
| Antigen | Antibody Source | Catalog/clone ID | Host | Isotype | Dilution | Retrieval | Positive Control Tissue | Negative Control Tissue |
|---|---|---|---|---|---|---|---|---|
| Vimentin | Dako | M7020 | M/m/aH | IgG2a | 1:150 | (0.5% in 5mM HCl) Pepsin, 40C°/15mints | Tonsil, Kidney | Internal stroma control |
| E Cadherin | Dako | M3612 | M/m/aH | IgG1(k) | 1:100 | 10mMTris Base, 1mM EDTA, 0.05% Tween20, pH 9.0 | Breast Cancer | Internal epithelial control |
| N Cadherin | Dako | M3613 | M/m/aH | IgG1(k) | 1:50 | 10mM Na Citrate / pH6.1 95C°/20mints | Heart | Internal stroma control |
| Ki67 | Dako | M7240 | M/m/aH | IgG1(k) | 1–50 | 10mM NaCitrate/0.05%Tween 20 pH6.1, 95C°/20mints | Tonsil | Benign tissue |
| Zeb1 | NovusBio | NBP1-88854 | R/p/aH | IgG | 1:250 | 1mM EDTA pH 8.0, 95C°/20mints | Breast Cancer | Normal breast Tissue |
| Snail/Slug | Abcam | Ab85936 | R/p/aH | IgG | 1:500 | 1mM EDTA pH 8.0, 95C°/20mints | Breast Cancer | Normal breast tissue |
| Twist | Abcam | Ab49254 | R/p/aH | IgG | 1:100 | 10mM NaCitrate / pH6.1 95C°/20mints | Testis | Normal Prostate |