Figure 2. Leptin regulates adiponectin expression in differentiated human white preadipocytes (HWP).
In-vitro treatment of differentiated HWP with increasing concentrations of leptin increases adiponectin mRNA (A) and protein (B) in a dose-dependent manner. However, leptin does not increase adiponectin secretion (C). Treatment of differentiated HWP with leptin antagonist decreases adiponectin mRNA (D), protein (E), and secretion (F). Data presented as mean ± SEM (n=4). *P ≤ 0.05 compared with control as determined by Wilcoxon rank-sum test. Total adiponectin: grey bars; high molecular weight adiponectin: black bars.