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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Mol Cancer Ther. 2015 Dec 4;15(2):264–275. doi: 10.1158/1535-7163.MCT-14-0315

Figure 1. Effects of low dose 2-DG on endothelial PI3K/mTOR and Erk pathways in vitro.

Figure 1

Starved HUVECs were stimulated with VEGF (a, b, c) or bFGF (d, e, f), treated with 0.6 mM 2-DG +/− 1mM Mannose, and incubated for 1 or 24 hrs. Lysates were used in standard Western blot procedure (methods). Membranes were then blotted against Akt pSer473, S6 pSer240/244, Erk pThr202/Tyr204, and GAPDH as loading control. Band intensity was analyzed by densitometry, normalized to GAPDH, and results displayed as means (+/− SEM) of relative protein density (percent) compared to untreated controls. GAPDH bands from figures 1. a, c and 1. d, f are similar as phosphoproteins were blotted from the same membranes. *p: NS. **: untreated vs 2-DG treated growth factor stimulated HUVECs. Pictures are representative of experiments performed at least in triplicate.

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