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HUVECs were plated in matrigel coated wells and treated with PBS (a) or 0.6 mM 2-DG (b). Tube formation was assessed and photographed at 2, 4, 8, and 18 hours. Scale bar 500μm. (c). Quantitative analysis of total tube length was performed as in methods. Histograms represent the average (+/− SEM) tube length (percent of control) of duplicate experiments, repeated at least twice.
