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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Mol Cancer Ther. 2015 Dec 4;15(2):264–275. doi: 10.1158/1535-7163.MCT-14-0315

Figure 7. Effects of GSK-3β inhibition on 2-DG induced endothelial AKT/ERK down regulation and apoptosis.

Figure 7

Growth factor stimulated HUVECs were treated with 0.6 mM 2-DG with or w/o the GSK-3β inhibitor BIO (5 nM), for 24 hours. Lysates were processed and Akt pSer473 and Erk pThr202/Tyr204, cleaved-Caspase-3 and cleaved-PARP were demonstrated using standard Western blot procedures (a). Densitometry analysis of bFGF (b) and VEGF (c) exposed HUVECs. GAPDH bands corresponding to pAKT and pERK (for each growth factor) are similar, as phosphoproteins were blotted from the same membrane *: untreated vs 2-DG treated growth factor stimulated HUVECs. ** 2-DG vs 2-DG+BIO treated, growth factor stimulated HUVECs.

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