Figure 8. In vivo effects of 2-DG on tumor angiogenesis and induction of unfolded protein response, and apoptosis.
LHBETATAG mice were treated with 2-DG or vehicle as described in methods. (a) Quantification of tumor microvessel density (total vasculature and neovessels) is presented as percent of total tumor area. Bars represent means +/− SEM of at least 4 independent samples per group. *p= 0.0079, **p= 0.0032 respectively. (b) Representative pictures of total and neovessels in control and 2-DG treated samples. (c) Tumor samples were stained for GRP78 and CD 105 (endoglin). Arrows represent CD105 and GRP78 positive areas. (d) In this experiment, mice were treated for 3 weeks as in methods. Samples were stained for apoptosis (TUNEL) and tumor endothelium (CD31). Representative pictures of experiments performed in quadruplicate. Arrows represent CD31 and TUNEL positive microvessels. Scale bars: 100 μm.