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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Nat Biotechnol. 2015 Nov;33(11):1193–1200. doi: 10.1038/nbt.3392

Figure 5. Self-organizing nephron formation in 3D culture.

Figure 5

(a, b) Whole-mount staining for CDH1, PODXL and LTL on day 28 (a) and 35 (b) using protocol in Figure 4a. Scale bar: 50 µm. (c, d) Representative immunohistochemistry in structures derived from hESCs and hiPSCs on day 21–28. n=5. Scale bars: 50 µm. CDH1: Cadherin-1 (E-cadherin). PODXL: Podocalyxin-like (Podocalyxin). LTL: lotus tetragonolobus lectin. AQP1: aquaporin1. NPHS1: Nephrin. UMOD: Uromodulin. (c) Low magnification. (d) High magnification. (e) Representative electron microscopy images of glomerulus-like and tubule regions of kidney organoids derived from hESCs. Middle panels represent higher magnification enlargement of the square-enclosed regions within left panels. n=5. Samples were taken at 21 days with the exception of the top right panel which was taken at day 18 and did not have transient CHIR treatment. Dotted lines: Bowman’s capsule. Arrows: foot process. Allow heads: tight-junction. Asterisks: mitochondria. Hashes: brush border-like structures. (f) Electron microscopy images of normal human kidneys showing foot processes (upper panel) and brush borders (lower panel).