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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Mol Cancer Ther. 2015 Dec 30;15(2):232–240. doi: 10.1158/1535-7163.MCT-15-0544

Figure 3.

Figure 3

A. Focused OBOC libraries structure; B. Focused library synthesis. a) 1. Swelling in water for 24 h; 2. Fmoc-OSu (0.04 equiv), Alloc-OSu (0.04 equiv.) and DIPEA, DCM/diethyl ether (55: 45), vigorously shaking 30min; b) Boc2O (3 equiv), DIPEA (6 equiv) in DMF, 2 h c): Pd(PPh3)4 (0.2 equiv), PhSiH3 (20 equiv) in DCM, twice (30 min each); d) Ac-Gly-OH (3 equiv.), DIC (3equiv.) and HOBt (3 equiv.), 2h; e) 1. 20% piperidine in DMF, 2 x 10 min.; 2. 50% TFA in DCM (2x30min); f) Split-mix combinatorial synthetic approach using Fmoc-L-amino acids; Four grams resin was split into two focused libraries after coupling of Fmoc-D-Cys(Trt)-OH; g) TFA: phenol: water: thioanisole: Tis (10:0.5:0.5:0.5:0.25, v/w/v/v/v), 3 h; h) 500mL mixture of water, acetic acid and DMSO (75:5:20, pH=6), shaking for 2 days. C. Chemical structure of LXW64 and Biotinylated LXW64.