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. Author manuscript; available in PMC: 2016 Oct 23.
Published in final edited form as: Circ Res. 2015 Sep 2;117(10):891–904. doi: 10.1161/CIRCRESAHA.115.306624

Figure 6. YAP-miR-206 signaling down regulates FoxP1 expression in CMs.

Figure 6

(A) CMs were transfected with luciferase reporter constructs harboring either wild-type (UTR) or mutated (UTRm) FoxP1 3′UTR sequences and then transduced with Ad-scrambled or Ad-miR-206. N=3. (B, C) CMs were transduced with Ad-LacZ, Ad-YAP, Ad-scrambled or Ad-miR-206 for 48 hours. Cells were harvested for immunoblot analyses with anti-FoxP1 antibody and either anti-actin or anti-α-tubulin antibodies. The results of densitometric analyses are also shown. N=4. (D, E) Tg-206-SPONGE, Tg-miR206/Tg-206-SPONGE and NTg mouse hearts were harvested for immunoblot analyses, using anti-FoxP1, anti-GFP and anti-actin antibodies. N=4.