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. 2016 Jan 25;13(1):68–76. doi: 10.7150/ijms.13016

Figure 7.

Figure 7

The effects of Krebs solution (pH 7.4 or pH 7.2) alone and Krebs solution (pH 7.4 or pH 7.2) in combination with SMOFlipid® emulsion (LE, 1.39%) on the activation of endothelial nitric oxide synthase (eNOS) at Ser1177 in human umbilical vein endothelial cells (HUVECs). HUVECs were treated for 10 min with either Krebs solution (pH 7.4 or pH 7.2) alone or Krebs solution (pH 7.4 or pH 7.2) in combination with SMOFlipid® emulsion (LE, 1.39%). The phosphorylation of eNOS at Ser1177 was investigated as described in the Methods section. Data are presented as the mean ± SD of five independent experiments. *P < 0.001 compared with the pH 7.4 Krebs solution. †P < 0.001 compared with the pH 7.2 Krebs solution alone. t-eNOS: total eNOS; p-eNOS: phosphorylated eNOS.