Figure 3.

The highest confidence network constructed with differentially methylated genes by proton beam treatment on the MCF-7 cell. The CpGs in which methylation level was significantly changed by proton beam treatment were analyzed through the Ingenuity pathway analysis software tool. The analysis indicated “Developmental Disorder, Hereditary Disorder, Metabolic Disease” as the network of highest confidence. Red-colored genes are hypermethylated in the proton beam treated cells, and green-colored genes indicate hypomethylation, with intensity signifying the magnitude of methylation change. Solid and dashed lines represent direct and indirect interactions, respectively, with each interaction supported by at least one literature reference.