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Green fluorescent protein (GFP)-immunoreactivity in Pdyn-IRES-Cre reporter mice (Pdyn-GFP, green) colocalizes with Pdyn mRNA in the lateral parabrachial nucleus (LPB) (Pdyn-FISH, in red). These examples are in the caudal dorsal-lateral parabrachial nucleus (approximate bregma −5.4 mm). Arrowheads indicate neurons with prominent colocalization. Scale bar is 50 μm.
