Table 1.

Approximate bregma levels for mouse lateral parabrachial nucleus analysis

Bregma	Neuroanatomic Landmarks
−4.9 mm	Far-rostral mesencephalic parabrachial nucleus (PB). PBreL (FoxP2+) is prominent; PBeL (FoxP2−) is not evident. The nucleus of the lateral lemniscus (NLL) is prominent as a large population of FoxP2+ neurons dorsolateral to the lateral parabrachial nucleus (LPB), intermingled with the auditory projections of the LL. The superior cerebellar peduncle is diffuse, turning rostro-medially toward its decussation.
−5.0 mm	Rostral mesencephalic PB. PB rostral-to-external lateral (PBreL) is prominent as an extensive population of FoxP2+ neurons surrounding the rostral tip of pB external lateral (PBeL), which is FoxP2−. NLL present. Pdyn-GFP+ neurons are scattered and few in number.
−5.1 mm	Middle mesencephalic PB. PBeL (FoxP2−) is prominent. PB dorsolateral (PBdl) (FoxP2+) is absent, but the rostral part of the central lateral subnucleus (FoxP2+ neurons; PB central lateral (PBcL) becomes prominent, as it merges rostrolaterally into PBreL.
−5.2 mm	Caudal mesencephalic PB. A thin isthmus of tissue at the pons-midbrain junction usually connects the caudal-ventral inferior colliculus to the LPB and pons. PBdL (FoxP2+) is present, merging into PBcL (also FoxP2+). PBeL is prominent (FoxP2−).
−5.3 mm	Rostral pontine PB. PBdL (FoxP2+) is prominent, along with a few cells in the caudal-most PBcL (FoxP2+). Caudal PBeL (FoxP2−) is present.
−5.4 mm	Middle pontine PB. Caudal PBdL (FoxP2+) is prominent. Caudal PBeL (FoxP2−) is thin or absent. FoxP2+ neurons are present at the extreme ventrolateral border of the LPB (lateral crescent and Kolliker-Fuse nuclei).
−5.5 mm	Far-caudal pontine PB. LPB is vanishingly thin, with very few neurons occupying the space between the superior cerebellar peduncle (scp) and ventral spinocerebellar tract, as they approach one another caudally (where the scp exits the cerebellum).