Table 3.

Relative abundances of bacterial genera in feces after β-glucan intervention.

Phyla	Taxa1	Diet2				SEM3	P-value
		Control	3 g LMW	5 g LMW	3 g HMW
Actinobacteria	g. Bifidobacterium	1.033	1.00	1.24	0.67	0.30	0.574
Bacteroidetes
	g. Bacteroides	5.96a	6.31ab	8.30ab	12.26b	1.60	0.0024
	f. Rikenellaceae	0.75	1.15	1.24	1.76	0.41	0.055
	g. Prevotella	0.48	0.54	1.46	1.84	0.71	0.095
	f. [Barnesiellaceae]	0.11	0.2	0.31	0.57	0.19	0.375
	g. Parabacteroides	0.17	0.14	0.18	0.23	0.054	0.625
	o. Bacteroidales	0.17ab	0.12a	0.21ab	0.24b	0.065	0.045
	f. Bacteroidaceae	0.079	0.13	0.12	0.15	0.14	0.325
Firmicutes
	g. Blautia	11.43	9.31	11.55	8.5	1.78	0.354
	g. Coprococcus	9.11	9.17	8.21	7.95	1.51	0.834
	g. Ruminococcus	6.18	6.83	5.82	4.8	0.92	0.214
	f. Lachnospiraceae unclassified	6.77	6.48	7.22	7.29	0.84	0.724
	f. Ruminococcaceae	4.63	4.32	3.34	4.04	0.78	0.414
	g. Faecalibacterium	5.34	4.71	3.92	4.32	1.57	0.175
	f. Ruminococcaceae	3.12	2.85	1.97	2.98	0.82	0.504
	f. Coprobacillaceae	2.45	2.91	3.31	2.91	0.66	0.554
	g. Roseburia	1.75	2.73	2.74	2.26	0.55	0.284
	g. [Ruminococcus]	3.43	2.86	2.99	2.76	0.58	0.494
	g. Oscillospira	1.56	1.62	1.29	1.48	0.48	0.824
	c. Clostridia unclassified	2.91	2.64	2.87	2.58	0.30	0.634
	o. Clostridiales	1.28	1.24	1.06	1.3	0.20	0.704
	g. Dialister	0.46	0.3	0.23	0.24	0.21	0.535
	f. Christensenellaceae	0.05	0.33	0.14	0.17	0.17	0.775
	g. Dorea	1.01	0.81	0.77	0.65	0.14	0.074
	g. Lachnospira	0.4	0.46	0.36	1.06	0.22	0.185
	f. Peptostreptococcaceae	0.34	0.67	0.47	0.26	0.19	0.474
	f. Lachnospiraceae	0.43	0.43	0.31	0.69	0.19	0.544
	f. Catabacteriaceae	0.42	0.61	0.35	0.81	0.50	0.274
	g. Catenibacterium	0.3	0.56	0.73	0.35	0.37	0.234
	c. Clostridia	0.14	0.17	0.13	0.17	0.04	0.634
	o. Clostridiales unclassified	0.098	0.27	0.28	0.11	0.14	0.864
	g. [Eubacterium]	0.15	0.12	0.11	0.11	0.12	0.984
	p. Firmicutes unclassified	0.25	0.24	0.26	0.21	0.032	0.394
	f. [Coprobacillaceae] unclassified	0.2	0.29	0.32	0.23	0.12	0.905
	f. Clostridiaceae unclassified	0.11	0.12	0.091	0.15	0.09	0.975
	g. Clostridium	0.097	0.14	0.084	0.12	0.08	0.965
	g. Turicibacter	0.056	0.079	0.043	0.066	0.07	0.985
	g. Streptococcus	0.27a	0.14ab	0.043ab	0.035b	0.07	0.025
Proteobacteria
	f. Enterobacteriaceae unclassified	0.12	0.011	0.18	0.54	0.19	0.095
Tenericutes
	o. RF39	0.082	0.062	0.085	0.17	0.058	0.525
Verrucomicrobia
	g. Akkermansia	0.14	0.38	0.19	0.39	0.17	0.285

^a,bMeans within the same row with different superscripts are different (P < 0.05).

¹Some sequences were only affiliated to phylum (p.), class (c.), order (o.), family (f.), or genus (g.) levels.

²Diets include: Wheat and rice control (Control), 3 g/d low molecular weight barley β-glucan (3 g LMW), 5 g/d low molecular weight barley β-glucan (5 g LMW), 3 g/d high molecular weight barley β-glucan (3 g HMW). Data are presented as mean values.

³SEM, Standard Error of Mean.

⁴P-values are shown for the diet effect between groups analyzed by mixed model ANCOVA with diet, period, period × diet as fixed factors.

⁵P-values are shown for the diet effect between groups analyzed by GLIMMIX procedure of SAS with diet, period, period × diet as fixed factors.