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. 2016 Feb 8;7:10656. doi: 10.1038/ncomms10656

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(a) The expression of Arf6 pathway proteins in ccRCC cell lines, assessed by immunoblotting analysis of total cell lysates using the indicated antibodies. Lysates of MDA-MB-231 cells were included for comparison. β-actin immunoblotting is shown as a control. (b) Matrigel invasion activities of 786-O cells in the presence of various ligands, as indicated. (c) Arf6 activity of 786-O cells on stimulation by LPA. (d) Requirement for Arf6, AMAP1 and EPB41L5 in LPA-induced Matrigel invasion of 786-O cells, as assessed by their gene silencing using specific siRNAs. (b,d) Error bars show the mean±s.e.m., n=3. *P<0.01. Statistical analyses were performed using analysis of variance.