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. 2016 Feb 8;7:10656. doi: 10.1038/ncomms10656

Figure 4. Involvement of EFA6A-C but not EFA6D in LPA-induced invasion and Arf6 activation of 786-O cells.

Figure 4

(a,b) Requirement for endogenous EFA6B, but not endogenous EFA6D, in LPA-induced Matrigel invasion (a) and Arf6 activation (b), as assessed by their gene silencing using specific siRNAs, as indicated. (c,d) EFA6A and EFA6C, expressed in 786-O cells instead of the endogenous EFA6B (EFA6A/shEFA6B cells and EFA6C/shEFA6B cells, respectively) promoted Matrigel invasion (c) and Arf6 activation (d) in response to LPA. shEFA6B cells and EFA6B/shEFA6B cells were included as controls. (a,b) siRNA with an irrelevant sequence (Irr) was included as a control. (a,c) error bars show the mean±s.e.m., n=3. *P<0.01. Statistical analyses were performed using analysis of variance.