Figure 6. Requirement for LPAR2 and the Arf6 mesechymal pathway in the lung metastasis.

786-O cells expressing a luciferase reporter gene and shRNA plasmids, each targeting EPB41L5 (shEPB41L5), EFA6B (shEFA6B) or LPAR2 (shLPAR2), were injected into tail veins of nude mice. Cells treated with shRNA plasmids bearing an irrelevant sequence (Irr) were included as a control. (a) Bioluminescence intensities of the chests of the mice, measured on day 0 and day 63, are shown. Results are shown as means±s.d., n=5. *P<0.01. Representative bioluminescence images of mice are shown on the right. Statistical analyses were performed using analysis of variance. (b) Representative images of lungs on day 63 post injection. Images of whole lungs (top) and hematoxylin and eosin staining of sections (bottom). Scale bar, 100 μm.