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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: J Neuroimmunol. 2015 Dec 12;291:18–27. doi: 10.1016/j.jneuroim.2015.12.004

Figure 4. Immunohistological analysis of microglial response in the cortex.

Figure 4

Double immunohistological staining for Iba1 (green) and MAP2 (red) proteins were examined in the cortex. Nuclei were stained with DAPI (blue). Upper panels: the contralateral (a, c) and ipsilateral (b, d) cortex of P9 (a, b) and P30 (c, d) brains at 9 days post-HI are compared. Lower panels: the contralateral (e, g) and ipsilateral (f, h) cortex of P9 (e, f) and P30 (g, h) brains at 9 days post-HI from minocycline-treated mice are compared. Insets: magnification (240X) of representative microglia demonstrating either ramified (arrow) or amoeboid (arrowhead) morphology.