Figure 5. Immunohistological analysis of microglial response in the striatum.
Double immunohistological staining for Iba1 (green) and MAP2 (red) proteins were examined in the striatum. Nuclei were stained with DAPI (blue). Upper panels: the contralateral (a, c) and ipsilateral (b, d) sides of P9 (a, b) and P30 (c, d) brains 9 days post-HI are compared. Lower panels: the contralateral (e, g) and ipsilateral (f, h) sides of P9 (e, f) and P30 (g, h) brains 9 days post-HI with minocycline treatment are compared. Insets: magnification (240X) of representative microglia demonstrating either ramified (arrow) or amoeboid (arrowhead) morphology.