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. 2016 Feb 10;6:20508. doi: 10.1038/srep20508

Figure 2. Kinetic analysis reveals a high affinity uptake for DNR into the TOV2223G cells.

Figure 2

Uptake rates were recorded for 2.5 min and quantified with the Fluoroskan Ascent microplate reader. The data were fitted to the following equation y = Vmax * x/(Km + x) by means of nonlinear regression using GraphPad Prism (GraphPad Software Inc, CA, USA) to determine Michaelis-Menten constant and maximum velocity. Results are representative of two separate experiments with standard errors.