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. 2016 Feb 10;6:20854. doi: 10.1038/srep20854

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Comparison of the classical scratch assay (a) and the novel agar diffusion scratch assay (b). In contrast to the scratch assay, cells were overlaid with agar in the agar diffusion scratch assay. Pictures are shown before and after 24 h of incubation. In the third column, viable cells were stained with Neutral Red (NR). The scale bar in the lower right picture represents 200 μm.