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. 2016 Feb 10;6:20922. doi: 10.1038/srep20922

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Copyright © 2016, Macmillan Publishers Limited

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Complexed pDCN and pIL-10 release from hydrogel in PBS over seven days (A). No significant difference (p > 0.05) in % human tenocyte viability was observed after transfection with complexed plasmids (pDCN, pIL-10, pDCN+IL-10) or plasmids (pDCN, pIL-10, pDCN+IL-10) in collagen hydrogel (B). The use of atelocollagen gel embedment of pDNA-polymer complexes to deliver eGFP showed improved green florescent protein expression at both 4 and 7 days post transfection, when compared with conventional direct pDNA-polymer complexes exposure to cells (C). Relative fluorescent intensity of eGFP after transfection revealed that transgene overexpression was extended for a period of 7 days (D). Note: *indicates p < 0.001.