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. 2016 Feb 10;6:20968. doi: 10.1038/srep20968

Figure 4. MiR218 regulates cell proliferation and differentiation by targeting sFRP2.

Figure 4

(A) Western blot analysis of proteins from CSCs transfected with sFRP2 shRNA, the miR218 inhibitor+sFRP2 shRNA and the miR218 inhibitor and subsequently cultured in differentiation media for 12 days. The protein profiles were normalized to β-actin. (B) The cells were stained with EdU and Hoechst 33342. (C) Cell cycle distribution of CSCs after transfection with sFRP2 shRNA, the miR218 inhibitor+sFRP2 shRNA and the miR218 inhibitor for 48 h.