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. 2016 Feb 10;6:20968. doi: 10.1038/srep20968

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Copyright © 2016, Macmillan Publishers Limited

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(A) Expression of Tcf-1 mRNA, (B) Lef-1 mRNA, (E) β-catenin mRNA and (G) CyclinD1 mRNA in CSCs transfected with the miR218 mimic and the niR218 inhibitor. The cells were analyzed on the indicated days. Relative expression levels were normalized to GADPH levels. (C) The miR218 mimic and miR218 inhibitor affect Wnt reporter activity. The WT Wnt TOPflash reporter was transfected into CSCs together with the mimic-control, miR218 mimic, inhibitor-control and miR218 inhibitor for 24 h. Relative luciferase activity was measured and plotted. (D) Wnt signaling activity in these cells during differentiation, examined by using Super Top flash. (F) Western blot analysis of the proteins from CSCs transfected with the miR218 mimic and the miR218 inhibitor and subsequently cultured in differentiation media for 12 days. The protein profiles were normalized to β-actin.