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. 2016 Feb 10;6:21577. doi: 10.1038/srep21577

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Copyright © 2016, Macmillan Publishers Limited

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At first, mature miRNAs are reverse transcribed using stem-loop primer (SLP). Forward primer (FP; miRNA specific, with 5′ overhang) and universal reverse primer (URP)³¹ are used to PCR-amplify the RT-product either through end-point PCR or quantitative PCR.