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. 2016 Feb 10;36(1):e00296. doi: 10.1042/BSR20150205

Figure 5. SNX27a down-regulates Wnt signalling.

Figure 5

(A) MCF7 cells were transfected with TOPflash and β-galactosidase plasmids, together with the indicated GFP vector or GFP-SNX27, and stimulated with 100 ng/ml Wnt3a. The cells then were processed for the detection of luciferase activity and β-galactosidase activity. The relative luciferase activity is normalized to the β-galactosidase activity. The results show that over-expression of SNX27 inhibits the Wnt regulated transcription activity. Statistical analysis was assessed by q test (Newman–Keuls test). (B) MCF7 cells expressing shRNA-ctrl or shRNA-SNX27 were transfected with TOPflash and β-galactosidase plasmids, and stimulated with 100 ng/ml Wnt3a. The transcription activity was detected similarly as mentioned above. The results demonstrate that the Wnt regulated transcription activity is greatly enhanced upon the depletion of SNX27. Statistical analysis was assessed by q test (Newman–Keuls test).