Table 2. Rate constants from ³¹P-NMR time courses of FtPPK2 assays.

			Nts
Experiment*	Initial [PolyP] /μM	Kinetic parameters†	ATP	ADP
1	500	k1 × 10−3/s−1	1.52±0.17	1.02±0.14
		R2	0.99	0.99
		ΔC/μM	1750±73	1880±36
		Initial rate/μM·s−1	2.65±0.41	1.915±0.30
2	16	k1 × 10−3/ s−1	0.769	ND‡
		R2	0.98	ND
		ΔC/μM	556	ND
		Initial rate/μM·s−1	0.450	ND

*Reactions also contained 150 nM FtPPK2, 2 mM nt, 2 mM CMP (internal standard) and 10% v/v ²H₂O.

†Time courses were fitted to a first order process: k₁, first order rate constant; R², goodness of fit; ΔC, calculated change in concentration of nt.

‡ND, not determined (a reliable fit could not be achieved).