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Multiple reaction monitoring transitions and corresponding collision energies for labeled and unlabeled adducted HSA peptides. Underlined lysine (K) indicates the site of MDI adduction.
| Adducted peptide | Monoisotopic transition pairs |
Optimized collision energies | |
|---|---|---|---|
| Precursor | Product | ||
| AFKAWAVAR | 622.3 (+2) | 673.1 | 16 |
| KQTALAELVK | 662.9 (+2) | 844.5 | 16 |
| YTK | 635.3 (+1) | 371.2 | 16 |
| KVPQVSTPTLVEVSR | 622.0 (+3) | 900.5 | 18 |
| 589.3 | 18 | ||
| 450.8 | 18 | ||
| KVPQVSTPTLVEV(+6)SR | 624.3 (+3) | 906.5 | 18 |
| 595.3 | 18 | ||
