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. 2016 Feb 10;6:20839. doi: 10.1038/srep20839

Table 1. Primers used in this study.

Primer Sequence (5′-3′) Enzyme site Purpose
P1 TTGCGGCCGCTCCGGAAGCTAATACGACTCACTATAGTTTTAAAACAGCCTGTGGG Not I CC024(1-2305) amplification
P2 CCCATATGGTTATAATGCCAG Nde I CC024(1-2305) amplification
P3 CCATATGGTATCAAACTAAC Nde I CC024(2298-6246) amplification
P4 CGTAGCATGCTTCCTC Sph I CC024(2298-6246) amplification
P5 GGAGGAAGCATGCTACGG Sph I CC024(6229-7409) amplification
P6 CGAGCTCTTTTTTTTTTTTTTTTTTTTTTTT Sac I CC024(6229-7409) amplification
P7 CTGTTTTATAACCCTTTTCCTAAGCAGCAACT None M1
P8 AGTTGCTGCTTAGGAAAAGGGTTATAAAACAG None M1
P9 CCCTTCCCCTAAGTAGCAACTTAGAAGCTT None M2
P10 AAGCTTCTAAGTTGCTACTTAGGGGAAGGG None M2
P11 AGCAACTTAGAAGCTCTATACAATCACGACCAA None M3
P12 TTGGTCGTGATTGTATAGAGCTTCTAAGTTGCT None M3
P13 CAGTCACGTCTTGGCCAAGCACTTCTGTATC None M4
P14 GATACAGAAGTGCTTGGCCAAGACGTGACTG None M4
P15 GAAAACGTTCGTTATCCGGCTAACTACT None M5
P16 AGTAGTTAGCCGGATAACGAACGTTTTC None M5
P17 CTAATACAGACATGGTGTGAAGAGTCTATTGAGCT None M6
P18 AGCTCAATAGACTCTTCACACCATGTCTGTATTAG None M6
P19 CGTGTTTCCTTTTATTTCTTATTGGCTGCTTATG None M7
P20 CATAAGCAGCCAATAAGAAATAAAAGGAAACACG None M7
P21 GGTGTCTAACAGAGCTGTTGTTTACCTATTTGTT None M8
P22 AACAAATAGGTAAACAACAGCTCTGTTAGACACC None M8
P23 TCGAGTTAAAACAGCCTGTGGGTT XhoI 5′UTR-pGL3-basic
P24 TTTCCTACAGTTAAGGAGCAATAT HindIII 5′UTR-pGL3-basic
P25 GTTAAAACAGCCTGTGGGTT XhoI 5′UTR-pGL3-basic
P26 AGCTTTTCCTACAGTTAAGGAGCAATAT HindIII 5′UTR-pGL3-basic
P27 GCGTCGACATGGATGCCGGTGTGAC SalI PCBP1
P28 TAGTTTAGCGGCCGCTACGCGTAATCTGGGACGTCGTAAGGGTAGCTGCACCCCATGCC NotI PCBP1
P29 GCGTCGACATGTACCCTTACGACGTCCCAGATTACGCGGAAACTGAACAGCCAG SalI hnRNPK
P30 ATAGTTTAGCGGCCGCTTAGAAAAACTTTCCAGAATACTGC NotI hnRNPK
P31 GCGTCGACATGTCTAAGTCAGAGTCTCCTAAAG SalI hnRNPA1
P32 CGGGATCCTTACGCGTAATCTGGGACGTCGTAAGGGTAAAATCTTCTGCCACTGCC BamHI hnRNPA1
P33 GCGTCGACTTAAAACAGCCTGTGGGTTG SalI 5′UTR-VR1012
P34 CGGGATCCTTCCTACAGTTAAGGAGCAAT BamHI 5′UTR-VR1012
P35 CTACTTCGAGAAACCTAGTAG None RT-qPCR CA16-5′UTR
P36 AGCCACTGTCACGGTCGC None RT-qPCR CA16-5′UTR
P37 CCCATCACCATCTTCCAGG None RT-qPCR GAPDH
P38 TTCTCCATGGTGGTGAAGAC None RT-qPCR GAPDH