Figure 5. Part of the promoter sequence of UBC and the primer sequence of UBC_3 (depicted bold and underlined).

The qPCR fragment is located around the transcription start site (CTG, italic underlined), starting from 31 bp upstream of the 5′-UTR (Untranslated Region) to 155 bp into this region and 553 bp upstream of the translation start codon (ATG, italic underlined). In the PCR fragment of the UBC_3 primers there are 14 CpG sites, of which one or more have differential methylation.