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Frontal chromatogram of increasing concentrations of
[3H]-epibatidine on the
α3β4α5 nicotinic receptor column, where: A
=240pM, B = 150 pM, C = 100 pM, D=80 pM and
E=60pM. Ammonium acetate buffer [10 mM, pH 7.4] was used
as eluent at a flow rate of 0.2 mL/min.