. Author manuscript; available in PMC: 2017 Jan 29.

Published in final edited form as: J Chromatogr A. 2015 Dec 29;1431:138–144. doi: 10.1016/j.chroma.2015.12.065

Table 1.

Binding affinities (Ki) determined using frontal displacement chromatography on both the α3β4 and α3β4α5 nAChRs using [³H]-epibatidine as the marker ligand.

Compound	α3β4α5	α3β4
Epibatidine	20.7 ± 12.6 pM (r²=0.96)	15.43 ± 10.4 pM (r²=0.89)
Nicotine	15.4 ± 9.6 nM (r²=0.98)	28.15 ± 14.6 nM (r²=0.97)
Cytisine	5.4 ± 3.5 nM (r²=0.89)	8.52 ± 5.7 nM (r²=0.95)
Nornicotine	20.8 ± 11.7nM (r²=0.88)	35.6± 18.9 nM (r²=0.98)
Anabasine	67.5± 25.8 nM (r²=0.92)	528.1 ± 135.6 nM (r²=0.86)