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. 2015 Sep 9;41(4):1112–1127. doi: 10.1038/npp.2015.250

Figure 4.

Figure 4

Chronic intermittent ethanol (CIE) exposure increases the amplitude, but not frequency (expressed as mean of the inter-event interval), of spontaneous excitatory postsynaptic currents (sEPSCs) and inhibitory postsynaptic currents (IPSCs) in lateral orbitofrontal cortex (lOFC) neurons. Representative traces of sEPSCs (top) and IPSCs (middle) from air- and CIE-treated (3D-WD) neurons are shown. Calibration bars (x=50 pA; y=0.5 s). A significant increase in amplitude (a; mean±SEM) but not frequency (b; expressed as inter-event interval) of AMPA-mediated sEPSCs was observed in CIE-exposed mice (one-way analysis of variance (ANOVA): F(3, 39)=2.889, p=0.048) and pairwise comparisons to control revealed significant increases at the 3-day (q=2.46, *p<0.05; n=17) and 10-day (q=2.55, **p<0.05; n=12) withdrawal (WD) time points. Increases in amplitude (c) but not frequency (d; expressed as inter-event interval) of sIPSCs were detected in 7-day (q=3.74, ** p<0.01; n=20) and 10-day (q=3.299, **p<0.01) WD animals. A trend toward an increase in the amplitude (e) but not frequency (f; expressed as inter-event interval) of miniature EPSCs (mEPSCs) was observed in CIE mice (one-way ANOVA: F(2, 18)=2.858, p=0.08), but pairwise comparisons revealed no significant differences between groups.