Table 1.

Breed, age, histopathology and disease diagnosis of sheep

Sheep ID	Breed	Origina	Age (years)	SGIb	AFBc	Tissue specific lesion graded	IS900 e	Diagnosisf
SH.139	Blackface	A	3	5	4	Severe	+, +	Multibacillary
SH.140	Blackface	A	3	7	4	Severe	+, +	Multibacillary
SH.146	Blackface x	B	2.5	5	4	Severe	+, +	Multibacillary
SH.190	Blackface	C	3	5	4	Severe	+, +	Multibacillary
SH.199	Blackface	C	2	6	4	Severe	+, +	Multibacillary
SH.204	Blackface	D	1.5	6	4	Severe	+, +	Multibacillary
SH.107	Blackface x	E	2.5	2.5	0	Mild	+, +	Paucibacillary
SH.147	Blackface x	B	2	3	0	Mild	+, +	Paucibacillary
SH.155	Blackface	F	3	2	0	Mild	+, +	Paucibacillary
SH.160	Blackface x	B	3	2.5	0	Mild	+, +	Paucibacillary
SH.188	Blackface x	B	4	4	1	Moderate	+, +	Paucibacillary
SH.205	Blackface	D	4	2	0	Mild	+, +	Paucibacillary
K207	Blackface	G	2.5	0	0	ND	−, −	Control
K208	Blackface	G	2.5	0	0	ND	−, −	Control
K213	Blackface	G	2.5	0	0	ND	−, −	Control
K224	Blackface	G	2.5	0	0	ND	−, −	Control
K227	Blackface	G	2.5	0	0	ND	−, −	Control
K229	Blackface	G	2.5	0	0	ND	−, −	Control

^aSource farms.

^bSeverity of granulomatous inflammation (SGI) grading: based on total number of epithelioid macrophages and leukocyte distribution patterns of the terminal ileum [5].

^cAcid fast bacteria (AFB) -; grading: grades 0–2 were defined as paucibacillary; grades 3–4 were defined as multibacillary observed in terminal ileum tissue [5].

^dTissue specific lesion grading: for each terminal ileum histological section, the sum of the points (SGI + AFB) was used to determine tissue-specific lesion grades. A severity of “none” was assigned to those with a lesion grade of 0, “mild” was assigned to a lesion grade of ≥2 and ≤3, “moderate” was assigned to those with a lesion grade of >3 and ≤5 and “severe” was assigned to a lesion grade of 6–11.

^e IS900 PCR result using each of the two primer sets.

^fDiagnosis: based on histopathological observations.