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. 2016 Feb 9;13:35. doi: 10.1186/s12974-016-0489-7

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© Tao et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Expression of ACE2 in DIZE pre-treated hRPE and ARPE-19 cells followed by LPS stimulation. The mRNA and protein expressions of ACE2 detected by real-time PCR and Western blotting showed that the ACE2 levels increased in the DIZE group and the DIZE+LPS group when compared with the untreated group and the LPS-treated group in both ARPE-19 cells (a) and hRPE (b). There was no significant difference between the untreated group and the LPS group. The band intensity of ACE2 was normalized to β-actin. The mRNA level of ACE2 was detected by real-time PCR in both ARPE-19 cells (c) and hRPE (d), which were transfected with ACE2-siRNA or the negative control scrambled siRNA. All data are expressed as mean ± SEM. (***p < 0.001, **p < 0.01, *p < 0.05, n = 4)