Fig. 3.
Evaluation of in vivo specific IgG antibody response measured as absorbance values elicited by CTB-adjuvanted combined vaccine candidate. Balb/C mice (n = 10 mice per group) were immunized subcutaneously with 109 CFU of formalin killed whole cell antigens. Antigens belonged to the above-mentioned five-diarrheagenic E. coli pathotypes,. Combined vaccine candidate consisted of formalin-killed whole cell of the main five pathotypes. Post-immunization blood samples were collected from mice groups weekly for 6 weeks. At week seven, mice were challenged with 106 CFU intraperitoneally and blood samples were collected 1 week after the challenge. Absorbance values of specific IgG antibody was measured for all seven intervals. Antibody absorbance values of combined vaccine candidate at selected time points compared to a EAEC antigens, b EPEC antigens, c EIEC antigens, d EHEC antigens and ETEC antigens. *p < 0.05, ** p < 0.001, ***p < 0.0001, and ****p < 0.00001, each bar represents mean ± standard deviation