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. 2016 Feb 9;15:34. doi: 10.1186/s12934-016-0433-7

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© Morschett et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Biovolume-specific optical density and average cell size of C. vulgaris at different physiological states. Depending on the physiological state observed, the cells significantly differ in their optical properties and cell size. Thereby, biomass determination across different states by either optical signals or cell number is distorted. Cell samples originated from phototrophic shaking flask cultivations. Error bars deviated from samples (biological replicates) of the respective fermentation phases (n ≥ 3)