Fig. 1.
Flow cytometric analysis results. a Forward scatter analysis (FSC-A) and side scatter analysis (SSC-A) of polymorphonuclear cells (neutrophils in gate P5) in a healthy control. b Negative outcome of phagocytosis of necrotic cell material (PNC) assay in a healthy control, where only 1.7 % of gated neutrophils are positive for propidium iodide (PI) and CD66. c Positive outcome of PNC assay in a patient affected by systemic lupus erythematosus, where 38.9 % of gated neutrophils are positive for PI and CD66