Fig. 3.

The SDS-PAGE profile of a separated proteins isolated from Lactobacillus johnsonii 151 in the presence of low molecular mass marker – 94, 67, 43, 30, 20.1, 14.4 kDa by continuous-elution electrophoresis (Prep-Cell apparatus Model 491 Bio-Rad). Samples are electrophoresed through a cylindrical gel. As molecules migrate through the gel matrix, they separate into bands. Individual bands migrate off the bottom of the gel where they pass directly into the patented elution chamber for collection. The resulting liquid fractions (2 ml) were pooled (5 fractions), dried and analyzed on SDS-PAGE. Gels were stained with Coomassie Brilliant Blue