Table 1.
Number of reads sequenced and mapped with Tophat. The number of unique mapping reads plus multimapping reads equals the total number of alignments. CK, H2O and IAA stand for imbibed tobacco seeds and control respectively; Numbers 0, 72 and 156 stand for germinated time
| Sample | Seed category | Total reads | Total mapped reads | Unique mapping reads | Reads mapped in proper pairs |
|---|---|---|---|---|---|
| Untreated-0 | Dormancy | 39,344,236 | 28,566,421(72.61 %) | 24,229,292(61.58 %) | 25,845,450 |
| H2O-0 | Imbibition | 39,795,012 | 35,183,360(88.41 %) | 21,537,407(66.58 %) | 29,258,962 |
| IAA-0 | Dormancy | 48,297,176 | 38,392,739(79.49 %) | 32,439,112(67.17 %) | 34,779,672 |
| Untreated-72 | Germination | 33,792,314 | 30,897,092(91.43 %) | 26,584,380(78.67 %) | 24,978,136 |
| H2O-72 | Germination | 35,593,768 | 31,962,893(89.80 %) | 27,456,500(77.14 %) | 26,621,694 |
| IAA-72 | Dormancy | 32,459,006 | 28,789,591(88.70 %) | 25,040,225(77.14 %) | 23,209,710 |
| IAA-156 | Germination | 33,769,804 | 29,877,097(88.47 %) | 25,821,822(76.46 %) | 24,613,498 |